ATR antibody

Catalog No. ABIN7111876

This Rabbit Polyclonal antibody specifically detects ATR in ELISA, WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATR antibody (ABIN7111876)

Target: ATR (ATR serine/threonine kinase (ATR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATR antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ATR Antibody

Purpose: ATR antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ataxia telangiectasia and Rad3 related

Isotype: IgG

Application Details

Application Notes: WB: 1:300-1:1000, IP: 1:200-1:1000, IHC: 1:50-1:500, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ATR

Target: ATR (ATR serine/threonine kinase (ATR))

Alternative Name: ATR

Background:

Synonyms: Serine/threonine-protein kinase ATR|Ataxia telangiectasia and Rad3-related protein|FRAP-related protein 1|ATR|FRP1

Background: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation(IR), ultraviolet light(UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

Molecular Weight: 250-290 kDa

Gene ID: 545

UniProt: Q13535

Pathways: Positive Regulation of Response to DNA Damage Stimulus